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000884076 1001_ $$0P:(DE-HGF)0$$aZaccagnini, Ludovica$$b0
000884076 245__ $$aIn silico/in vitro screening and hit evaluation identified new phenothiazine anti-prion derivatives
000884076 260__ $$aAmsterdam [u.a.]$$bElsevier71544$$c2020
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000884076 520__ $$aPrion diseases or transmissible spongiform encephalopathies (TSEs) are a group of rare neurodegenerative disorders. TSEs are characterized by the accumulation of prions (PrPSc) that represent pathological isoforms of the physiological cellular prion protein PrPC. Although the conversion of PrPC to PrPSc is still not completely understood, blocking this process may lead to develop new therapies. Here, we have generated a pharmacophore model, based on anti-prion molecules reported in literature to be effective in phenotypic assay. The model was used to conduct a virtual screen of commercial compound databases that selected a small library of ten compounds. These molecules were then screened in mouse neuroblastoma cell line chronically infected with prions (ScN2a) after excluding neurotoxicity. 1 has been identified as the therapeutic hit on the basis of the following evidence: chronic treatments of ScN2a cells using 1 eliminate PrPSc loaded in both Western blotting analysis and Real-Time Quaking-Induced Conversion (RT-QuIC) assay. We also proposed the mechanism of action of 1 by which it has the ability to bind PrPC and consequentially blocks prion conversion. Herein we describe the results of these efforts.
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000884076 7001_ $$0P:(DE-Juel1)145921$$aRossetti, Giulia$$b1
000884076 7001_ $$0P:(DE-HGF)0$$aTran, Thanh Hoa$$b2
000884076 7001_ $$0P:(DE-HGF)0$$aSalzano, Giulia$$b3
000884076 7001_ $$0P:(DE-HGF)0$$aGandini, Annachiara$$b4
000884076 7001_ $$0P:(DE-HGF)0$$aColini Baldeschi, Arianna$$b5
000884076 7001_ $$0P:(DE-HGF)0$$aBolognesi, Maria Laura$$b6
000884076 7001_ $$0P:(DE-Juel1)145614$$aCarloni, Paolo$$b7
000884076 7001_ $$0P:(DE-HGF)0$$aLegname, Giuseppe$$b8$$eCorresponding author
000884076 77318 $$2Crossref$$3journal-article$$a10.1016/j.ejmech.2020.112295$$bElsevier BV$$d2020-06-01$$p112295$$tEuropean Journal of Medicinal Chemistry$$v196$$x0223-5234$$y2020
000884076 773__ $$0PERI:(DE-600)2005170-0$$a10.1016/j.ejmech.2020.112295$$gVol. 196, p. 112295 -$$p112295$$tEuropean journal of medicinal chemistry$$v196$$x0223-5234$$y2020
000884076 8564_ $$uhttps://juser.fz-juelich.de/record/884076/files/Journal%20Pre-Proof.pdf$$yPublished on 2020-04-08. Available in OpenAccess from 2022-04-08.
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