Journal Article

FZJ-2020-03077

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png In silico/in vitro screening and hit evaluation identified new phenothiazine anti-prion derivatives

Zaccagnini, L. ; Rossetti, G.FZJ* ; Tran, T. H. ; Salzano, G. ; Gandini, A. ; Colini Baldeschi, A. ; Bolognesi, M. L. ; Carloni, P.FZJ* ; Legname, G. (Corresponding author)

2020
Elsevier71544 Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/26976  doi:10.1016/j.ejmech.2020.112295

Abstract: Prion diseases or transmissible spongiform encephalopathies (TSEs) are a group of rare neurodegenerative disorders. TSEs are characterized by the accumulation of prions (PrPSc) that represent pathological isoforms of the physiological cellular prion protein PrPC. Although the conversion of PrPC to PrPSc is still not completely understood, blocking this process may lead to develop new therapies. Here, we have generated a pharmacophore model, based on anti-prion molecules reported in literature to be effective in phenotypic assay. The model was used to conduct a virtual screen of commercial compound databases that selected a small library of ten compounds. These molecules were then screened in mouse neuroblastoma cell line chronically infected with prions (ScN2a) after excluding neurotoxicity. 1 has been identified as the therapeutic hit on the basis of the following evidence: chronic treatments of ScN2a cells using 1 eliminate PrPSc loaded in both Western blotting analysis and Real-Time Quaking-Induced Conversion (RT-QuIC) assay. We also proposed the mechanism of action of 1 by which it has the ability to bind PrPC and consequentially blocks prion conversion. Herein we describe the results of these efforts.

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Contributing Institute(s):

1. Computational Biomedicine (IAS-5)
2. Computational Biomedicine (INM-9)
3. Jülich Supercomputing Center (JSC)

Research Program(s):

1. 574 - Theory, modelling and simulation (POF3-574) (POF3-574)
2. 511 - Computational Science and Mathematical Methods (POF3-511) (POF3-511)

Appears in the scientific report 2020

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Database coverage:
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