%0 Journal Article
%A Brands, Stefanie
%A Brass, Hannah U. C.
%A Klein, Andreas
%A Pietruszka, Jörg
%A Ruff, Anna Joëlle
%A Schwaneberg, Ulrich
%T A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
%J Chemical communications
%V 56
%N 61
%@ 1364-548X
%C Cambridge
%I Soc.
%M FZJ-2020-03838
%P 8631 - 8634
%D 2020
%X A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ 32588862
%U <Go to ISI:>//WOS:000554386800012
%R 10.1039/D0CC02181D
%U https://juser.fz-juelich.de/record/885455