Journal Article

FZJ-2020-03838

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

Brands, S.RWTH* ; Brass, H. U. C.FZJ* ; Klein, A.FZJ* ; Pietruszka, J.FZJ* ; Ruff, A. J.RWTH* ; Schwaneberg, U. (Corresponding author)RWTH*

2020
Soc. Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/26906  doi:10.1039/D0CC02181D

Abstract: A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.

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Contributing Institute(s):

1. Institut für Bioorganische Chemie (HHUD) (IBOC)
2. Biotechnologie (IBG-1)

Research Program(s):

1. 583 - Innovative Synergisms (POF3-583) (POF3-583)

Appears in the scientific report 2020

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Database coverage:
; ; Allianz-Lizenz / DFG ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; Index Chemicus ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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