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000885455 1001_ $$0P:(DE-HGF)0$$aBrands, Stefanie$$b0
000885455 245__ $$aA colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
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000885455 520__ $$aA colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
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000885455 7001_ $$0P:(DE-Juel1)169140$$aBrass, Hannah U. C.$$b1
000885455 7001_ $$0P:(DE-Juel1)157813$$aKlein, Andreas$$b2
000885455 7001_ $$0P:(DE-Juel1)128906$$aPietruszka, Jörg$$b3$$ufzj
000885455 7001_ $$0P:(DE-HGF)0$$aRuff, Anna Joëlle$$b4
000885455 7001_ $$0P:(DE-HGF)0$$aSchwaneberg, Ulrich$$b5$$eCorresponding author
000885455 773__ $$0PERI:(DE-600)1472881-3$$a10.1039/D0CC02181D$$gVol. 56, no. 61, p. 8631 - 8634$$n61$$p8631 - 8634$$tChemical communications$$v56$$x1364-548X$$y2020
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