TY  - JOUR
AU  - Brands, Stefanie
AU  - Brass, Hannah U. C.
AU  - Klein, Andreas
AU  - Pietruszka, Jörg
AU  - Ruff, Anna Joëlle
AU  - Schwaneberg, Ulrich
TI  - A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
JO  - Chemical communications
VL  - 56
IS  - 61
SN  - 1364-548X
CY  - Cambridge
PB  - Soc.
M1  - FZJ-2020-03838
SP  - 8631 - 8634
PY  - 2020
AB  - A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
LB  - PUB:(DE-HGF)16
C6  - 32588862
UR  - <Go to ISI:>//WOS:000554386800012
DO  - DOI:10.1039/D0CC02181D
UR  - https://juser.fz-juelich.de/record/885455
ER  -