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@ARTICLE{Brands:885455,
      author       = {Brands, Stefanie and Brass, Hannah U. C. and Klein, Andreas
                      and Pietruszka, Jörg and Ruff, Anna Joëlle and
                      Schwaneberg, Ulrich},
      title        = {{A} colourimetric high-throughput screening system for
                      directed evolution of prodigiosin ligase {P}ig{C}},
      journal      = {Chemical communications},
      volume       = {56},
      number       = {61},
      issn         = {1364-548X},
      address      = {Cambridge},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-03838},
      pages        = {8631 - 8634},
      year         = {2020},
      abstract     = {A colourimetric high-throughput screening system was
                      established for directed evolution of prodigiosin ligase
                      PigC. The two-step system consists of a colony prescreening
                      test and a subsequent photometric 96-well plate assay.
                      Screening PigC epPCR libraries in Pseudomonas putida
                      revealed a PigC variant that achieved a 2.9× increased
                      yield of prodiginine derivatives.},
      cin          = {IBOC / IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBOC-20090406 / I:(DE-Juel1)IBG-1-20101118},
      pnm          = {583 - Innovative Synergisms (POF3-583)},
      pid          = {G:(DE-HGF)POF3-583},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {32588862},
      UT           = {WOS:000554386800012},
      doi          = {10.1039/D0CC02181D},
      url          = {https://juser.fz-juelich.de/record/885455},
}