Hauptseite > Publikationsdatenbank > A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC > print

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024 7 _ |a 10.1039/D0CC02181D
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100 1 _ |a Brands, Stefanie
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245 _ _ |a A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
260 _ _ |a Cambridge
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520 _ _ |a A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
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700 1 _ |a Brass, Hannah U. C.
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700 1 _ |a Klein, Andreas
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700 1 _ |a Pietruszka, Jörg
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700 1 _ |a Ruff, Anna Joëlle
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700 1 _ |a Schwaneberg, Ulrich
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773 _ _ |a 10.1039/D0CC02181D
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856 4 _ |y Published on 2020-06-18. Available in OpenAccess from 2021-06-18.
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