TY  - EJOUR
AU  - Costantini, Irene
AU  - Baria, Enrico
AU  - Sorelli, Michele
AU  - Matuschke, Felix
AU  - Giardini, Francesco
AU  - Menzel, Miriam
AU  - Mazzamuto, Giacomo
AU  - Silvestri, Ludovico
AU  - Cicchi, Riccardo
AU  - Amunts, Katrin
AU  - Axer, Markus
AU  - Pavone, Francesco Saverio
TI  - MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes
M1  - FZJ-2020-04004
PY  - 2020
AB  - Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluorescence imaging of myelinated fibers. We demonstrate its broad applicability by performing mesoscopic reconstruction at sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in Control and Reeler mouse’s hippocampal sections.
LB  - PUB:(DE-HGF)25
DO  - DOI:10.1101/2020.07.28.225011
UR  - https://juser.fz-juelich.de/record/885673
ER  -