Preprint

FZJ-2020-04004

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png MAGIC: A label-free fluorescence method for 3D high-resolution reconstruction of myelinated fibers in large volumes

Costantini, I. (Corresponding author) ; Baria, E. ; Sorelli, M. ; Matuschke, F.FZJ* ; Giardini, F. ; Menzel, M.FZJ* ; Mazzamuto, G. ; Silvestri, L. ; Cicchi, R. ; Amunts, K.FZJ* ; Axer, M.FZJ* ; Pavone, F. S.

2020

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Please use a persistent id in citations: http://hdl.handle.net/2128/25892  doi:10.1101/2020.07.28.225011

Abstract: Analyzing the structure of neuronal fibers with single axon resolution, in large volumes, remains an unresolved challenge in connectomics. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a simple tissue preparation method to perform label-free fluorescence imaging of myelinated fibers. We demonstrate its broad applicability by performing mesoscopic reconstruction at sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in Control and Reeler mouse’s hippocampal sections.

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Contributing Institute(s):

1. Strukturelle und funktionelle Organisation des Gehirns (INM-1)

Research Program(s):

1. 571 - Connectivity and Activity (POF3-571) (POF3-571)
2. HBP SGA2 - Human Brain Project Specific Grant Agreement 2 (785907) (785907)
3. HBP SGA3 - Human Brain Project Specific Grant Agreement 3 (945539) (945539)

Appears in the scientific report 2020

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Database coverage:

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