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@ARTICLE{Costantini:885673,
author = {Costantini, Irene and Baria, Enrico and Sorelli, Michele
and Matuschke, Felix and Giardini, Francesco and Menzel,
Miriam and Mazzamuto, Giacomo and Silvestri, Ludovico and
Cicchi, Riccardo and Amunts, Katrin and Axer, Markus and
Pavone, Francesco Saverio},
title = {{MAGIC}: {A} label-free fluorescence method for 3{D}
high-resolution reconstruction of myelinated fibers in large
volumes},
reportid = {FZJ-2020-04004},
year = {2020},
abstract = {Analyzing the structure of neuronal fibers with single axon
resolution, in large volumes, remains an unresolved
challenge in connectomics. Here, we propose MAGIC (Myelin
Autofluorescence imaging by Glycerol Induced Contrast
enhancement), a simple tissue preparation method to perform
label-free fluorescence imaging of myelinated fibers. We
demonstrate its broad applicability by performing mesoscopic
reconstruction at sub-micron resolution of mouse, rat,
monkey, and human brain samples and by quantifying the
different fiber organization in Control and Reeler mouse’s
hippocampal sections.},
cin = {INM-1},
cid = {I:(DE-Juel1)INM-1-20090406},
pnm = {571 - Connectivity and Activity (POF3-571) / HBP SGA2 -
Human Brain Project Specific Grant Agreement 2 (785907) /
HBP SGA3 - Human Brain Project Specific Grant Agreement 3
(945539)},
pid = {G:(DE-HGF)POF3-571 / G:(EU-Grant)785907 /
G:(EU-Grant)945539},
typ = {PUB:(DE-HGF)25},
doi = {10.1101/2020.07.28.225011},
url = {https://juser.fz-juelich.de/record/885673},
}