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@ARTICLE{Minopoli:888502,
author = {Minopoli, Antonio and Sakač, Nikola and Lenyk, Bohdan and
Campanile, Raffaele and Mayer, Dirk and Offenhäusser,
Andreas and Velotta, Raffaele and Della Ventura, Bartolomeo},
title = {{LSPR}-based colorimetric immunosensor for rapid and
sensitive 17β-estradiol detection in tap water},
journal = {Sensors and actuators / B},
volume = {308},
issn = {0925-4005},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2020-04966},
pages = {127699 -},
year = {2020},
abstract = {We propose a highly sensitive immunosensor based on the
Localized Surface Plasmon Resonance (LSPR) for
17β-estradiol (E2) quantification in water. E2 molecules
are recognized by polyclonal antibodies immobilized onto
gold nanoparticles (AuNPs) and act as linkers that cause
nanoparticles aggregation. This leads to the change in the
optical properties of the solution visible even by naked
eyes. The aggregates were characterized by Dynamic Light
Scattering (DLS) and Scanning Transmission Electron
Microscopy (STEM) that provided an accurate assessment of
the inter-particle distance. The finite-difference
time-domain (FDTD) method applied to a Mie problem like
workspace allowed us to describe the optical behaviour of
the AuNP aggregates with excellent agreement between the
experimental and numerical results. The limit of detection
(LOD), without any preconcentration step, is 3 pg/mL (11
pM), whereas the detection range extends over five decades
up to 105 pg/mL. The proposed E2 immunosensor was tested in
tap water, where no significant cross-reaction signal was
detected by similar molecules (testosterone, progesterone,
estrone and estriol). The device described here represents a
significant improvement of low E2 levels determination in
terms of affordability, time and measuring simplicity,
making it suitable for environmental applications.},
cin = {IBI-3},
ddc = {620},
cid = {I:(DE-Juel1)IBI-3-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000511146700026},
doi = {10.1016/j.snb.2020.127699},
url = {https://juser.fz-juelich.de/record/888502},
}