Journal Article

FZJ-2020-04966

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png LSPR-based colorimetric immunosensor for rapid and sensitive 17β-estradiol detection in tap water

Minopoli, A.FZJ* ; Sakač, N. ; Lenyk, B.FZJ* ; Campanile, R.Extern* ; Mayer, D. (Corresponding author)FZJ* ; Offenhäusser, A.FZJ* ; Velotta, R.Extern* ; Della Ventura, B.Extern*

2020
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/34150  doi:10.1016/j.snb.2020.127699

Abstract: We propose a highly sensitive immunosensor based on the Localized Surface Plasmon Resonance (LSPR) for 17β-estradiol (E2) quantification in water. E2 molecules are recognized by polyclonal antibodies immobilized onto gold nanoparticles (AuNPs) and act as linkers that cause nanoparticles aggregation. This leads to the change in the optical properties of the solution visible even by naked eyes. The aggregates were characterized by Dynamic Light Scattering (DLS) and Scanning Transmission Electron Microscopy (STEM) that provided an accurate assessment of the inter-particle distance. The finite-difference time-domain (FDTD) method applied to a Mie problem like workspace allowed us to describe the optical behaviour of the AuNP aggregates with excellent agreement between the experimental and numerical results. The limit of detection (LOD), without any preconcentration step, is 3 pg/mL (11 pM), whereas the detection range extends over five decades up to 105 pg/mL. The proposed E2 immunosensor was tested in tap water, where no significant cross-reaction signal was detected by similar molecules (testosterone, progesterone, estrone and estriol). The device described here represents a significant improvement of low E2 levels determination in terms of affordability, time and measuring simplicity, making it suitable for environmental applications.

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2020

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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