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@ARTICLE{Schmitz:888659,
      author       = {Schmitz, Julian and Täuber, Sarah and Westerwalbesloh,
                      Christoph and Lieres, Eric and Noll, Thomas and Grünberger,
                      Alexander},
      title        = {{D}evelopment and application of a cultivation platform for
                      mammalian suspension cell lines with single‐cell
                      resolution},
      journal      = {Biotechnology $\&$ bioengineering},
      volume       = {118},
      number       = {2},
      issn         = {1097-0290},
      address      = {New York, NY [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2020-05101},
      pages        = {992-1005},
      year         = {2021},
      abstract     = {In bioproduction processes, cellular heterogeneity can
                      cause unpredictable process outcomes or even provoke process
                      failure. Still, cellular heterogeneity is not examined
                      systematically in bioprocess research and development. One
                      reason for this shortcoming is the applied average bulk
                      analyses, which are not able to detect cell‐to‐cell
                      differences. In this study, we present a microfluidic tool
                      for mammalian single‐cell cultivation (MaSC) of suspension
                      cells. The design of our platform allows cultivation in
                      highly controllable environments. As a model system, Chinese
                      hamster ovary cells (CHO‐K1) were cultivated over 150 h.
                      Growth behavior was analyzed on a single‐cell level and
                      resulted in growth rates between 0.85 and 1.16 day−1. At
                      the same time, heterogeneous growth and division behavior,
                      for example, unequal division time, as well as rare cellular
                      events like polynucleation or reversed mitosis were
                      observed, which would have remained undetected in a standard
                      population analysis based on average measurements.
                      Therefore, MaSC will open the door for systematic
                      single‐cell analysis of mammalian suspension cells.
                      Possible fields of application represent basic research
                      topics like cell‐to‐cell heterogeneity, clonal
                      stability, pharmaceutical drug screening, and stem cell
                      research, as well as bioprocess related topics such as media
                      development and novel scale‐down approaches.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {33200818},
      UT           = {WOS:000592056500001},
      doi          = {10.1002/bit.27627},
      url          = {https://juser.fz-juelich.de/record/888659},
}