TY  - JOUR
AU  - Borlinghaus, Jan
AU  - Bolger, Anthony
AU  - Schier, Christina
AU  - Vogel, Alexander
AU  - Usadel, Björn
AU  - Gruhlke, Martin CH
AU  - Slusarenko, Alan J
TI  - Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin
JO  - Life science alliance
VL  - 3
IS  - 5
SN  - 2575-1077
CY  - Heidelberg
PB  - EMBO Press
M1  - FZJ-2021-01208
SP  - e202000670 -
PY  - 2020
N1  - Rheinisch-Westfälische Technische Hochschule Aachen UniversityBundesministerium für Bildung und Forschung (BMBF) grant 031A536.
AB  - The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant Pseudomonas fluorescens (PfAR-1), which was isolated from garlic, conferred allicin resistance to Pseudomonas syringae and even to Escherichia coli. Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in PfAR-1, achieved through horizontal gene transfer.
LB  - PUB:(DE-HGF)16
C6  - 32234751
UR  - <Go to ISI:>//WOS:000538076900009
DO  - DOI:10.26508/lsa.202000670
UR  - https://juser.fz-juelich.de/record/890800
ER  -