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| 001 | 890800 | ||
| 005 | 20230111074330.0 | ||
| 024 | 7 | _ | |a 10.26508/lsa.202000670 |2 doi |
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| 100 | 1 | _ | |a Borlinghaus, Jan |0 0000-0001-6052-2108 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin |
| 260 | _ | _ | |a Heidelberg |c 2020 |b EMBO Press |
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| 500 | _ | _ | |a Rheinisch-Westfälische Technische Hochschule Aachen UniversityBundesministerium für Bildung und Forschung (BMBF) grant 031A536. |
| 520 | _ | _ | |a The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant Pseudomonas fluorescens (PfAR-1), which was isolated from garlic, conferred allicin resistance to Pseudomonas syringae and even to Escherichia coli. Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in PfAR-1, achieved through horizontal gene transfer. |
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| 700 | 1 | _ | |a Gruhlke, Martin CH |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Slusarenko, Alan J |0 0000-0003-3311-5878 |b 6 |
| 773 | _ | _ | |a 10.26508/lsa.202000670 |g Vol. 3, no. 5, p. e202000670 - |0 PERI:(DE-600)2948687-7 |n 5 |p e202000670 - |t Life science alliance |v 3 |y 2020 |x 2575-1077 |
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