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| 001 | 893282 | ||
| 005 | 20240619091616.0 | ||
| 024 | 7 | _ | |a 10.3390/s21124144 |2 doi |
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| 037 | _ | _ | |a FZJ-2021-02672 |
| 082 | _ | _ | |a 620 |
| 100 | 1 | _ | |a Reinartz, Ines |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Structural Analysis of a Genetically Encoded FRET Biosensor by SAXS and MD Simulations |
| 260 | _ | _ | |a Basel |c 2021 |b MDPI |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1639985730_10728 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Inspired by the modular architecture of natural signaling proteins, ligand binding proteins are equipped with two fluorescent proteins (FPs) in order to obtain Förster resonance energy transfer (FRET)-based biosensors. Here, we investigated a glucose sensor where the donor and acceptor FPs were attached to a glucose binding protein using a variety of different linker sequences. For three resulting sensor constructs the corresponding glucose induced conformational changes were measured by small angle X-ray scattering (SAXS) and compared to recently published single molecule FRET results (Höfig et al., ACS Sensors, 2018). For one construct which exhibits a high change in energy transfer and a large change of the radius of gyration upon ligand binding, we performed coarse-grained molecular dynamics simulations for the ligand-free and the ligand-bound state. Our analysis indicates that a carefully designed attachment of the donor FP is crucial for the proper transfer of the glucose induced conformational change of the glucose binding protein into a well pronounced FRET signal change as measured in this sensor construct. Since the other FP (acceptor) does not experience such a glucose induced alteration, it becomes apparent that only one of the FPs needs to have a well-adjusted attachment to the glucose binding protein. |
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| 700 | 1 | _ | |a Sarter, Mona |0 P:(DE-Juel1)171618 |b 1 |
| 700 | 1 | _ | |a Otten, Julia |0 P:(DE-Juel1)165764 |b 2 |
| 700 | 1 | _ | |a Höfig, Henning |0 P:(DE-Juel1)185813 |b 3 |u fzj |
| 700 | 1 | _ | |a Pohl, Martina |0 P:(DE-Juel1)131522 |b 4 |
| 700 | 1 | _ | |a Schug, Alexander |0 P:(DE-Juel1)173652 |b 5 |
| 700 | 1 | _ | |a Stadler, Andreas M. |0 P:(DE-Juel1)140278 |b 6 |
| 700 | 1 | _ | |a Fitter, Jörg |0 P:(DE-Juel1)131961 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.3390/s21124144 |g Vol. 21, no. 12, p. 4144 - |0 PERI:(DE-600)2052857-7 |n 12 |p 4144 - |t Sensors |v 21 |y 2021 |x 1424-8220 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/893282/files/sensors-21-04144-v2.pdf |y OpenAccess |
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