% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Nassiri:894308, author = {Nassiri, Farshad and Wang, Justin Z and Singh, Olivia and Karimi, Shirin and Dalcourt, Tatyana and Ijad, Nazanin and Pirouzmand, Neda and Ng, Ho-Keung and Saladino, Andrea and Pollo, Bianca and Dimeco, Francesco and Yip, Stephen and Gao, Andrew and Aldape, Kenneth D and Zadeh, Gelareh and Aldape, Kenneth and Au, Karolyn and Barnholtz-Sloan, Jill and Behling, Felix and Bi, Wenya and Brastianos, Priscilla and Butowski, Nicholas and Brodie, Chaya and Cohen-Gadol, Aaron and Couce, Marta and Dimeco, Francesco and Drummond, Kate and Dunn, Ian and Cohen-Gadol, Aaron and Galanis, Eva and Galldiks, Norbert and Giannini, Caterina and Goldbrunner, Roland and Hanemann, Oliver and Herold-Mende, Christel and Horbinski, Craig and Huang, Raymond and Javadpour, Mohsen and Jenkinson, Michael and Jungk, Christine and Kaufmann, Timothy and Krischek, Boris and Kurz, Sylvia and Lachance, Daniel and Lafougere, Christian and Lamszus, Katrin and Lee, Ian and Malta, Tathiana and Makarenko, Serge and Mawrin, Christian and McDermott, Michael and Millward, Christopher and Moliterno-Gunel, Jennifer and Morokoff, Andrew and Nassiri, Farshad and Ng, H. K. and Noushmehr, Houtan and Perry, Arie and Poisson, Laila and Pollo, Bianco and Ragunathan, Aditya and Raleigh, David and Renovanz, Mirjam and Ricklefs, Franz and Sahm, Felix and Saladino, Andrea and Santacroce, Antonio and Santarius, Thomas and Schichor, Christian and Schimdt, Nils and Schittenhelm, Jens and Selman, Warren and Shih, Helen and Snyder, Jim and Snuderl, Matja and Sloan, Andrew and Suppiah, Suganth and Sulman, Erik and Tabatabai, Ghazaleh and Tatagiba, Marcos and Timmer, Marcos and Tonn, Joerg-Christian and Von Deimling, Andreas and Vogelbaum, Michael and Walbert, Tobias and Wang, Justin and Wen, Patrick and Westphal, Manfred and Yip, Stephen and Zadeh, Gelareh}, title = {{L}oss of {H}3{K}27me3 in meningiomas}, journal = {Neuro-Oncology}, volume = {23}, number = {8}, issn = {1523-5866}, address = {Oxford}, publisher = {Oxford Univ. Press}, reportid = {FZJ-2021-03168}, pages = {1282 - 1291}, year = {2021}, abstract = {BackgroundThere is a critical need for objective and reliable biomarkers of outcome in meningiomas beyond WHO classification. Loss of H3K27me3 has been reported as a prognostically unfavorable alteration in meningiomas. We sought to independently evaluate the reproducibility and prognostic value of H3K27me3 loss by immunohistochemistry (IHC) in a multicenter study.MethodsIHC staining for H3K27me3 and analyses of whole slides from 181 meningiomas across three centers was performed. Staining was analyzed by dichotomization into loss and retained immunoreactivity, and using a 3-tiered scoring system in 151 cases with clear staining. Associations of grouping with outcome were performed using Kaplan-Meier survival estimates.ResultsA total of 21 of 151 tumors $(13.9\%)$ demonstrated complete loss of H3K27me3 staining in tumor with retained endothelial staining. Overall, loss of H3K27me3 portended a worse outcome with shorter times to recurrence in our cohort, particularly for WHO grade 2 tumors which were enriched in our study. There were no differences in recurrence-free survival (RFS) for WHO grade 3 patients with retained vs loss of H3K27me3. Scoring by a 3-tiered system did not add further insights into the prognostic value of this H3K27me3 loss. Overall, loss of H3K27me3 was not independently associated with RFS after controlling for WHO grade, extent of resection, sex, age, and recurrence status of tumor on multivariable Cox regression analysis.ConclusionsLoss of H3K27me3 identifies a subset of WHO grade 2 and possibly WHO grade 1 meningiomas with increased recurrence risk. Pooled analyses of a larger cohort of samples with standardized reporting of clinical definitions and staining patterns are warranted.}, cin = {INM-3}, ddc = {610}, cid = {I:(DE-Juel1)INM-3-20090406}, pnm = {5252 - Brain Dysfunction and Plasticity (POF4-525)}, pid = {G:(DE-HGF)POF4-5252}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:33970242}, UT = {WOS:000685248000012}, doi = {10.1093/neuonc/noab036}, url = {https://juser.fz-juelich.de/record/894308}, }