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000894460 1001_ $$0P:(DE-Juel1)168172$$aTenhaef, Niklas$$b0
000894460 245__ $$aMicroaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum
000894460 260__ $$aWeinheim$$bWiley-VCH$$c2021
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000894460 520__ $$aBackgroundLignocellulosic biomass is the most abundant raw material on earth. Its efficient use for novel bio-based materials is essential for an emerging bioeconomy. Possible building blocks for such materials are the key TCA-cycle intermediates α-ketoglutarate and succinate. These organic acids have a wide range of potential applications, particularly in use as monomers for established or novel biopolymers. Recently, Corynebacterium glutamicum was successfully engineered and evolved towards an improved utilization of d-xylose via the Weimberg pathway, yielding the strain WMB2evo. The Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of d-xylose to α-ketoglutarate and a shortcut route to succinate as co-product in a one-pot process.Methods and ResultsC. glutamicum WMB2evo was grown under dynamic microaerobic conditions on d-xylose, leading to the formation of comparably high amounts of succinate and only small amounts of α-ketoglutarate. Subsequent carbon isotope labeling experiments verified the targeted production route for both products in C. glutamicum WMB2evo. Fed-batch process development was initiated and the effect of oxygen supply and feeding strategy for a growth-decoupled co-production of α-ketoglutarate and succinate were studied in detail. The finally established fed-batch production process resulted in the formation of 78.4 mmol L−1 (11.45 g L−1) α-ketoglutarate and 96.2 mmol L−1 (11.36 g L−1) succinate.ConclusionThe developed one-pot process represents a promising approach for the combined supply of bio-based α-ketoglutarate and succinate. Future work will focus on tailor-made down-stream processing of both organic acids from the fermentation broth to enable their application as building blocks in chemical syntheses. Alternatively, direct conversion of one or both acids via whole-cell or cell-free enzymatic approaches can be envisioned; thus, extending the network of value chains starting from cheap and renewable d-xylose.AbstractThe Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of xylose to α-ketoglutarate and a shortcut route to succinate as established platform chemical. In this study, we employed the recently engineered and evolved strain C. glutamicum WMB2evo to establish a one-pot cultivation process for co-production of α-ketoglutarate and succinate from xylose. 
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000894460 7001_ $$0P:(DE-Juel1)161539$$aKappelmann, Jannick$$b1
000894460 7001_ $$0P:(DE-Juel1)173876$$aEich, Arabel$$b2
000894460 7001_ $$0P:(DE-Juel1)173753$$aWeiske, Marc$$b3
000894460 7001_ $$0P:(DE-Juel1)176823$$aBrieß, Lisette$$b4
000894460 7001_ $$0P:(DE-Juel1)166290$$aBrüsseler, Christian$$b5
000894460 7001_ $$0P:(DE-Juel1)144031$$aMarienhagen, Jan$$b6
000894460 7001_ $$0P:(DE-Juel1)129076$$aWiechert, Wolfgang$$b7
000894460 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b8$$eCorresponding author
000894460 773__ $$0PERI:(DE-600)2214038-4$$a10.1002/biot.202100043$$gp. 2100043 -$$n9$$p2100043 -$$tBiotechnology journal$$v16$$x1860-7314$$y2021
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