TY  - JOUR
AU  - Tenhaef, Niklas
AU  - Kappelmann, Jannick
AU  - Eich, Arabel
AU  - Weiske, Marc
AU  - Brieß, Lisette
AU  - Brüsseler, Christian
AU  - Marienhagen, Jan
AU  - Wiechert, Wolfgang
AU  - Noack, Stephan
TI  - Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum
JO  - Biotechnology journal
VL  - 16
IS  - 9
SN  - 1860-7314
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2021-03236
SP  - 2100043 -
PY  - 2021
N1  - Biotechnologie 1
AB  - BackgroundLignocellulosic biomass is the most abundant raw material on earth. Its efficient use for novel bio-based materials is essential for an emerging bioeconomy. Possible building blocks for such materials are the key TCA-cycle intermediates α-ketoglutarate and succinate. These organic acids have a wide range of potential applications, particularly in use as monomers for established or novel biopolymers. Recently, Corynebacterium glutamicum was successfully engineered and evolved towards an improved utilization of d-xylose via the Weimberg pathway, yielding the strain WMB2evo. The Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of d-xylose to α-ketoglutarate and a shortcut route to succinate as co-product in a one-pot process.Methods and ResultsC. glutamicum WMB2evo was grown under dynamic microaerobic conditions on d-xylose, leading to the formation of comparably high amounts of succinate and only small amounts of α-ketoglutarate. Subsequent carbon isotope labeling experiments verified the targeted production route for both products in C. glutamicum WMB2evo. Fed-batch process development was initiated and the effect of oxygen supply and feeding strategy for a growth-decoupled co-production of α-ketoglutarate and succinate were studied in detail. The finally established fed-batch production process resulted in the formation of 78.4 mmol L−1 (11.45 g L−1) α-ketoglutarate and 96.2 mmol L−1 (11.36 g L−1) succinate.ConclusionThe developed one-pot process represents a promising approach for the combined supply of bio-based α-ketoglutarate and succinate. Future work will focus on tailor-made down-stream processing of both organic acids from the fermentation broth to enable their application as building blocks in chemical syntheses. Alternatively, direct conversion of one or both acids via whole-cell or cell-free enzymatic approaches can be envisioned; thus, extending the network of value chains starting from cheap and renewable d-xylose.AbstractThe Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of xylose to α-ketoglutarate and a shortcut route to succinate as established platform chemical. In this study, we employed the recently engineered and evolved strain C. glutamicum WMB2evo to establish a one-pot cultivation process for co-production of α-ketoglutarate and succinate from xylose. 
LB  - PUB:(DE-HGF)16
C6  - 34089621
UR  - <Go to ISI:>//WOS:000662896100001
DO  - DOI:10.1002/biot.202100043
UR  - https://juser.fz-juelich.de/record/894460
ER  -