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@ARTICLE{Tenhaef:894460,
      author       = {Tenhaef, Niklas and Kappelmann, Jannick and Eich, Arabel
                      and Weiske, Marc and Brieß, Lisette and Brüsseler,
                      Christian and Marienhagen, Jan and Wiechert, Wolfgang and
                      Noack, Stephan},
      title        = {{M}icroaerobic growth‐decoupled production of
                      α‐ketoglutarate and succinate from xylose in a one‐pot
                      process using {C}orynebacterium glutamicum},
      journal      = {Biotechnology journal},
      volume       = {16},
      number       = {9},
      issn         = {1860-7314},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2021-03236},
      pages        = {2100043 -},
      year         = {2021},
      note         = {Biotechnologie 1},
      abstract     = {BackgroundLignocellulosic biomass is the most abundant raw
                      material on earth. Its efficient use for novel bio-based
                      materials is essential for an emerging bioeconomy. Possible
                      building blocks for such materials are the key TCA-cycle
                      intermediates α-ketoglutarate and succinate. These organic
                      acids have a wide range of potential applications,
                      particularly in use as monomers for established or novel
                      biopolymers. Recently, Corynebacterium glutamicum was
                      successfully engineered and evolved towards an improved
                      utilization of d-xylose via the Weimberg pathway, yielding
                      the strain WMB2evo. The Weimberg pathway enables a
                      carbon-efficient C5-to-C5 conversion of d-xylose to
                      α-ketoglutarate and a shortcut route to succinate as
                      co-product in a one-pot process.Methods and ResultsC.
                      glutamicum WMB2evo was grown under dynamic microaerobic
                      conditions on d-xylose, leading to the formation of
                      comparably high amounts of succinate and only small amounts
                      of α-ketoglutarate. Subsequent carbon isotope labeling
                      experiments verified the targeted production route for both
                      products in C. glutamicum WMB2evo. Fed-batch process
                      development was initiated and the effect of oxygen supply
                      and feeding strategy for a growth-decoupled co-production of
                      α-ketoglutarate and succinate were studied in detail. The
                      finally established fed-batch production process resulted in
                      the formation of 78.4 mmol L−1 (11.45 g L−1)
                      α-ketoglutarate and 96.2 mmol L−1 (11.36 g L−1)
                      succinate.ConclusionThe developed one-pot process represents
                      a promising approach for the combined supply of bio-based
                      α-ketoglutarate and succinate. Future work will focus on
                      tailor-made down-stream processing of both organic acids
                      from the fermentation broth to enable their application as
                      building blocks in chemical syntheses. Alternatively, direct
                      conversion of one or both acids via whole-cell or cell-free
                      enzymatic approaches can be envisioned; thus, extending the
                      network of value chains starting from cheap and renewable
                      d-xylose.AbstractThe Weimberg pathway enables a
                      carbon-efficient C5-to-C5 conversion of xylose to
                      α-ketoglutarate and a shortcut route to succinate as
                      established platform chemical. In this study, we employed
                      the recently engineered and evolved strain C. glutamicum
                      WMB2evo to establish a one-pot cultivation process for
                      co-production of α-ketoglutarate and succinate from
                      xylose.},
      cin          = {IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {34089621},
      UT           = {WOS:000662896100001},
      doi          = {10.1002/biot.202100043},
      url          = {https://juser.fz-juelich.de/record/894460},
}