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| Home > Publications database > Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum > print |
| 001 | 894460 | ||
| 005 | 20230303201752.0 | ||
| 024 | 7 | _ | |a 10.1002/biot.202100043 |2 doi |
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| 100 | 1 | _ | |a Tenhaef, Niklas |0 P:(DE-Juel1)168172 |b 0 |
| 245 | _ | _ | |a Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum |
| 260 | _ | _ | |a Weinheim |c 2021 |b Wiley-VCH |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1631518810_22188 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a Biotechnologie 1 |
| 520 | _ | _ | |a BackgroundLignocellulosic biomass is the most abundant raw material on earth. Its efficient use for novel bio-based materials is essential for an emerging bioeconomy. Possible building blocks for such materials are the key TCA-cycle intermediates α-ketoglutarate and succinate. These organic acids have a wide range of potential applications, particularly in use as monomers for established or novel biopolymers. Recently, Corynebacterium glutamicum was successfully engineered and evolved towards an improved utilization of d-xylose via the Weimberg pathway, yielding the strain WMB2evo. The Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of d-xylose to α-ketoglutarate and a shortcut route to succinate as co-product in a one-pot process.Methods and ResultsC. glutamicum WMB2evo was grown under dynamic microaerobic conditions on d-xylose, leading to the formation of comparably high amounts of succinate and only small amounts of α-ketoglutarate. Subsequent carbon isotope labeling experiments verified the targeted production route for both products in C. glutamicum WMB2evo. Fed-batch process development was initiated and the effect of oxygen supply and feeding strategy for a growth-decoupled co-production of α-ketoglutarate and succinate were studied in detail. The finally established fed-batch production process resulted in the formation of 78.4 mmol L−1 (11.45 g L−1) α-ketoglutarate and 96.2 mmol L−1 (11.36 g L−1) succinate.ConclusionThe developed one-pot process represents a promising approach for the combined supply of bio-based α-ketoglutarate and succinate. Future work will focus on tailor-made down-stream processing of both organic acids from the fermentation broth to enable their application as building blocks in chemical syntheses. Alternatively, direct conversion of one or both acids via whole-cell or cell-free enzymatic approaches can be envisioned; thus, extending the network of value chains starting from cheap and renewable d-xylose.AbstractThe Weimberg pathway enables a carbon-efficient C5-to-C5 conversion of xylose to α-ketoglutarate and a shortcut route to succinate as established platform chemical. In this study, we employed the recently engineered and evolved strain C. glutamicum WMB2evo to establish a one-pot cultivation process for co-production of α-ketoglutarate and succinate from xylose. |
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| 700 | 1 | _ | |a Kappelmann, Jannick |0 P:(DE-Juel1)161539 |b 1 |
| 700 | 1 | _ | |a Eich, Arabel |0 P:(DE-Juel1)173876 |b 2 |
| 700 | 1 | _ | |a Weiske, Marc |0 P:(DE-Juel1)173753 |b 3 |
| 700 | 1 | _ | |a Brieß, Lisette |0 P:(DE-Juel1)176823 |b 4 |
| 700 | 1 | _ | |a Brüsseler, Christian |0 P:(DE-Juel1)166290 |b 5 |
| 700 | 1 | _ | |a Marienhagen, Jan |0 P:(DE-Juel1)144031 |b 6 |
| 700 | 1 | _ | |a Wiechert, Wolfgang |0 P:(DE-Juel1)129076 |b 7 |
| 700 | 1 | _ | |a Noack, Stephan |0 P:(DE-Juel1)129050 |b 8 |e Corresponding author |
| 773 | _ | _ | |a 10.1002/biot.202100043 |g p. 2100043 - |0 PERI:(DE-600)2214038-4 |n 9 |p 2100043 - |t Biotechnology journal |v 16 |y 2021 |x 1860-7314 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/894460/files/Tenhaef%20et%20al%202021%20Biotechnol%20J%20online.pdf |y OpenAccess |
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