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@ARTICLE{lc:902906,
      author       = {Ölçücü, Gizem and Klaus, Oliver and Jaeger, Karl-Erich
                      and Drepper, Thomas and Krauss, Ulrich},
      title        = {{E}merging {S}olutions for in {V}ivo {B}iocatalyst
                      {I}mmobilization: {T}ailor-{M}ade {C}atalysts for
                      {I}ndustrial {B}iocatalysis},
      journal      = {ACS sustainable chemistry $\&$ engineering},
      volume       = {9},
      number       = {27},
      issn         = {2168-0485},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2021-04661},
      pages        = {8919 - 8945},
      year         = {2021},
      abstract     = {In industry, enzymes are often immobilized to generate more
                      stable enzyme preparations that are easier to store, handle,
                      and recycle for repetitive use. Traditionally, enzymes are
                      bound to inorganic carrier materials, which requires
                      case-to-case optimization and incurs additional labor and
                      costs. Therefore, with the advent of rational protein design
                      strategies as part of bottom-up synthetic biology
                      approaches, numerous immobilization methods have been
                      developed that enable the one-step production and
                      immobilization of enzymes onto biogenic carrier materials
                      often directly within the production host, which we here
                      refer to as in vivo immobilization. As a result, nano- to
                      micro-meter-sized functionalized biomaterials, or
                      biologically produced enzyme immobilizates, are obtained
                      that can directly be used for synthetic purposes. In this
                      Perspective, we provide an overview over established and
                      recently emerging in vivo enzyme immobilization methods,
                      with special emphasis on their applicability for
                      (industrial) biocatalysis. For each approach, we present
                      fundamental working principles as well as advantages and
                      limitations guiding future research avenues toward
                      sustainable applications in the bioindustry.},
      cin          = {IMET / IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IMET-20090612 / I:(DE-Juel1)IBG-1-20101118},
      pnm          = {2172 - Utilization of renewable carbon and energy sources
                      and engineering of ecosystem functions (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2172},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000674165800002},
      doi          = {10.1021/acssuschemeng.1c02045},
      url          = {https://juser.fz-juelich.de/record/902906},
}