Journal Article

FZJ-2021-04661

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Emerging Solutions for in Vivo Biocatalyst Immobilization: Tailor-Made Catalysts for Industrial Biocatalysis

Ölçücü, G.FZJ* ; Klaus, O.FZJ* ; Jaeger, K.-E.FZJ* ; Drepper, T.FZJ* ; Krauss, U. (Corresponding author)FZJ*

2021
ACS Publ. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/29222  doi:10.1021/acssuschemeng.1c02045

Abstract: In industry, enzymes are often immobilized to generate more stable enzyme preparations that are easier to store, handle, and recycle for repetitive use. Traditionally, enzymes are bound to inorganic carrier materials, which requires case-to-case optimization and incurs additional labor and costs. Therefore, with the advent of rational protein design strategies as part of bottom-up synthetic biology approaches, numerous immobilization methods have been developed that enable the one-step production and immobilization of enzymes onto biogenic carrier materials often directly within the production host, which we here refer to as in vivo immobilization. As a result, nano- to micro-meter-sized functionalized biomaterials, or biologically produced enzyme immobilizates, are obtained that can directly be used for synthetic purposes. In this Perspective, we provide an overview over established and recently emerging in vivo enzyme immobilization methods, with special emphasis on their applicability for (industrial) biocatalysis. For each approach, we present fundamental working principles as well as advantages and limitations guiding future research avenues toward sustainable applications in the bioindustry.

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Contributing Institute(s):

1. Institut für Molekulare Enzymtechnologie (HHUD) (IMET)
2. Biotechnologie (IBG-1)

Research Program(s):

1. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2021

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Database coverage:
; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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