TY  - JOUR
AU  - Longo, M.
AU  - Heigl, R. J.
AU  - Schrader, Tobias Erich
TI  - Real-time investigation of Lysozyme crystallization kinetics: A neutron diffraction study
JO  - Journal of crystal growth
VL  - 576
SN  - 0022-0248
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - FZJ-2021-05120
SP  - 126362
PY  - 2021
AB  - The isothermal crystallization kinetics of hen-egg-white Lysozyme has been investigatedby means of a time-resolved neutron diffraction experiment for almost 3 days,starting from a supersaturated solution of Lysozyme (30 mg/ml, 3 wt % NaCl, pD 4.75at 298 K) until the growth of crystals, in order to have complementary informationabout the crystallization kinetics. The temporal evolution of the intensity of the Braggpeaks, observed in the neutron diffraction images when a single crystal appears, hasbeen studied. Simultaneously, the analysis of the small angle neutron scattering curvesfrom the Lysozyme solution, during the crystallization process, has been performed.A correlated behaviour between the decrease of the Lysozyme concentration and theincrease of the crystallization fraction was observed. The crystallization kinetics wasdescribed by means of the Johnson-Mehl-Avrami-Kolmogorov model and parameterscompatible with our previous Lysozyme crystallization study have been found.The Lysozyme crystallization under constant neutron flux underlines the known strengthof neutrons in studying biological samples without causing radiation damage.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000712269600001
DO  - DOI:10.1016/j.jcrysgro.2021.126362
UR  - https://juser.fz-juelich.de/record/903441
ER  -