Journal Article

FZJ-2021-05120

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Real-time investigation of Lysozyme crystallization kinetics: A neutron diffraction study

Longo, M.FZJ* ; Heigl, R. J. ; Schrader, T. E. (Corresponding author)FZJ*

2021
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/29452  doi:10.1016/j.jcrysgro.2021.126362

Abstract: The isothermal crystallization kinetics of hen-egg-white Lysozyme has been investigatedby means of a time-resolved neutron diffraction experiment for almost 3 days,starting from a supersaturated solution of Lysozyme (30 mg/ml, 3 wt % NaCl, pD 4.75at 298 K) until the growth of crystals, in order to have complementary informationabout the crystallization kinetics. The temporal evolution of the intensity of the Braggpeaks, observed in the neutron diffraction images when a single crystal appears, hasbeen studied. Simultaneously, the analysis of the small angle neutron scattering curvesfrom the Lysozyme solution, during the crystallization process, has been performed.A correlated behaviour between the decrease of the Lysozyme concentration and theincrease of the crystallization fraction was observed. The crystallization kinetics wasdescribed by means of the Johnson-Mehl-Avrami-Kolmogorov model and parameterscompatible with our previous Lysozyme crystallization study have been found.The Lysozyme crystallization under constant neutron flux underlines the known strengthof neutrons in studying biological samples without causing radiation damage.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. JCNS-4 (JCNS-4)
3. Heinz Maier-Leibnitz Zentrum (MLZ)
4. Neutronenstreuung (JCNS-1)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. BIODIFF: Diffractometer for large unit cells (NL1)

Appears in the scientific report 2021

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Electronics and Telecommunications Collection ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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