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@ARTICLE{Krugmann:903794,
author = {Krugmann, Benjamin and Koutsioumpas, Alexandros and Haris,
Luman and Micciulla, Samantha and Lairez, Didier and
Radulescu, Aurel and Förster, Stephan and Stadler, Andreas
M.},
title = {{A}dhesion {P}rocess of {B}iomimetic {M}yelin {M}embranes
{T}riggered by {M}yelin {B}asic {P}rotein},
journal = {Frontiers in Chemistry},
volume = {9},
issn = {2296-2646},
address = {Lausanne},
publisher = {Frontiers Media},
reportid = {FZJ-2021-05429},
pages = {631277},
year = {2021},
abstract = {The myelin sheath—a multi-double-bilayer membrane wrapped
around axons—is an essential part of the nervous system
which enables rapid signal conduction. Damage of this
complex membrane system results in demyelinating diseases
such as multiple sclerosis (MS). The process in which myelin
is generated in vivo is called myelination. In our study, we
investigated the adhesion process of large unilamellar
vesicles with a supported membrane bilayer that was coated
with myelin basic protein (MBP) using time-resolved neutron
reflectometry. Our aim was to mimic and to study the
myelination process of membrane systems having either a
lipid-composition resembling that of native myelin or that
of the standard animal model for experimental autoimmune
encephalomyelitis (EAE) which represents MS-like conditions.
We were able to measure the kinetics of the partial
formation of a double bilayer in those systems and to
characterize the scattering length density profiles of the
initial and final states of the membrane. The kinetics could
be modeled using a random sequential adsorption simulation.
By using a free energy minimization method, we were able to
calculate the shape of the adhered vesicles and to determine
the adhesion energy per MBP. For the native membrane the
resulting adhesion energy per MBP is larger than that of the
EAE modified membrane type. Our observations might help in
understanding myelination and especially remyelination—a
process in which damaged myelin is repaired—which is a
promising candidate for treatment of the still mostly
incurable demyelinating diseases such as MS.},
cin = {JCNS-1 / JCNS-4 / JCNS-FRM-II / MLZ / IBI-8},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-4-20201012 /
I:(DE-Juel1)JCNS-FRM-II-20110218 / I:(DE-588b)4597118-3 /
I:(DE-Juel1)IBI-8-20200312},
pnm = {6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ)
(POF4-6G4) / 632 - Materials – Quantum, Complex and
Functional Materials (POF4-632)},
pid = {G:(DE-HGF)POF4-6G4 / G:(DE-HGF)POF4-632},
experiment = {EXP:(DE-MLZ)MARIA-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34017815},
UT = {WOS:000651217900001},
doi = {10.3389/fchem.2021.631277},
url = {https://juser.fz-juelich.de/record/903794},
}