Journal Article

FZJ-2021-05429

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Adhesion Process of Biomimetic Myelin Membranes Triggered by Myelin Basic Protein

Krugmann, B.FZJ* ; Koutsioumpas, A.FZJ* ; Haris, L.FZJ* ; Micciulla, S. ; Lairez, D. ; Radulescu, A.FZJ* ; Förster, S.FZJ* ; Stadler, A. M. (Corresponding author)FZJ*

2021
Frontiers Media Lausanne

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Please use a persistent id in citations: http://hdl.handle.net/2128/29588  doi:10.3389/fchem.2021.631277

Abstract: The myelin sheath—a multi-double-bilayer membrane wrapped around axons—is an essential part of the nervous system which enables rapid signal conduction. Damage of this complex membrane system results in demyelinating diseases such as multiple sclerosis (MS). The process in which myelin is generated in vivo is called myelination. In our study, we investigated the adhesion process of large unilamellar vesicles with a supported membrane bilayer that was coated with myelin basic protein (MBP) using time-resolved neutron reflectometry. Our aim was to mimic and to study the myelination process of membrane systems having either a lipid-composition resembling that of native myelin or that of the standard animal model for experimental autoimmune encephalomyelitis (EAE) which represents MS-like conditions. We were able to measure the kinetics of the partial formation of a double bilayer in those systems and to characterize the scattering length density profiles of the initial and final states of the membrane. The kinetics could be modeled using a random sequential adsorption simulation. By using a free energy minimization method, we were able to calculate the shape of the adhered vesicles and to determine the adhesion energy per MBP. For the native membrane the resulting adhesion energy per MBP is larger than that of the EAE modified membrane type. Our observations might help in understanding myelination and especially remyelination—a process in which damaged myelin is repaired—which is a promising candidate for treatment of the still mostly incurable demyelinating diseases such as MS.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. Neutronenstreuung (JCNS-1)
2. JCNS-4 (JCNS-4)
3. JCNS-FRM-II (JCNS-FRM-II)
4. Heinz Maier-Leibnitz Zentrum (MLZ)
5. Neutronenstreuung und biologische Materie (IBI-8)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. MARIA: Magnetic reflectometer with high incident angle (NL5N)

Appears in the scientific report 2021

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