%0 Journal Article
%A Mukhina, Tetiana
%A Gerelli, Yuri
%A Hemmerle, Arnaud
%A Koutsioumpas, Alexandros
%A Kovalev, Kirill
%A Teulon, Jean-Marie
%A Pellequer, Jean-Luc
%A Daillant, Jean
%A Charitat, Thierry
%A Fragneto, Giovanna
%T Insertion and activation of functional Bacteriorhodopsin in a floating bilayer
%J Journal of colloid and interface science
%V 597
%@ 0021-9797
%C Amsterdam [u.a.]
%I Elsevier
%M FZJ-2021-05478
%P 370 - 382
%D 2021
%X The proton pump transmembrane protein bacteriorhodopsin was successfully incorporated into planar floating lipid bilayers in gel and fluid phases, by ap- plying a detergent-mediated incorporation method. The method was optimized on single supported bilayers by using quartz crystal microbalance, atomic force and fluorescence microscopy techniques. Neutron and X-ray reflectometry were used on both single and floating bilayers with the aim of determining the struc- ture and composition of this membrane-protein system before and after protein reconstitution at sub-nanometer resolution. Lipid bilayer integrity and pro- tein activity were preserved upon the reconstitution process. Reversible struc- tural modifications of the membrane, induced by the bacteriorhodopsin func- tional activity triggered by visible light, were observed and characterized at the nanoscale.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:33894545
%U <Go to ISI:>//WOS:000653055500004
%R 10.1016/j.jcis.2021.03.155
%U https://juser.fz-juelich.de/record/903845