Journal Article

FZJ-2021-05478

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Insertion and activation of functional Bacteriorhodopsin in a floating bilayer

Mukhina, T. ; Gerelli, Y. ; Hemmerle, A. ; Koutsioumpas, A.FZJ* ; Kovalev, K. ; Teulon, J.-M. ; Pellequer, J.-L. ; Daillant, J. ; Charitat, T. (Corresponding author) ; Fragneto, G. (Corresponding author)

2021
Elsevier Amsterdam [u.a.]

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/29569  doi:10.1016/j.jcis.2021.03.155

Abstract: The proton pump transmembrane protein bacteriorhodopsin was successfully incorporated into planar floating lipid bilayers in gel and fluid phases, by ap- plying a detergent-mediated incorporation method. The method was optimized on single supported bilayers by using quartz crystal microbalance, atomic force and fluorescence microscopy techniques. Neutron and X-ray reflectometry were used on both single and floating bilayers with the aim of determining the struc- ture and composition of this membrane-protein system before and after protein reconstitution at sub-nanometer resolution. Lipid bilayer integrity and pro- tein activity were preserved upon the reconstitution process. Reversible struc- tural modifications of the membrane, induced by the bacteriorhodopsin func- tional activity triggered by visible light, were observed and characterized at the nanoscale.

Keyword(s): Health and Life (1st) ; Biology (2nd)

---

Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Streumethoden (JCNS-2)
3. JCNS-4 (JCNS-4)
4. Heinz Maier-Leibnitz Zentrum (MLZ)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. MARIA: Magnetic reflectometer with high incident angle (NL5N)

Appears in the scientific report 2021

---

Database coverage:
; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---