TY  - JOUR
AU  - Mukhina, Tetiana
AU  - Gerelli, Yuri
AU  - Hemmerle, Arnaud
AU  - Koutsioumpas, Alexandros
AU  - Kovalev, Kirill
AU  - Teulon, Jean-Marie
AU  - Pellequer, Jean-Luc
AU  - Daillant, Jean
AU  - Charitat, Thierry
AU  - Fragneto, Giovanna
TI  - Insertion and activation of functional Bacteriorhodopsin in a floating bilayer
JO  - Journal of colloid and interface science
VL  - 597
SN  - 0021-9797
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - FZJ-2021-05478
SP  - 370 - 382
PY  - 2021
AB  - The proton pump transmembrane protein bacteriorhodopsin was successfully incorporated into planar floating lipid bilayers in gel and fluid phases, by ap- plying a detergent-mediated incorporation method. The method was optimized on single supported bilayers by using quartz crystal microbalance, atomic force and fluorescence microscopy techniques. Neutron and X-ray reflectometry were used on both single and floating bilayers with the aim of determining the struc- ture and composition of this membrane-protein system before and after protein reconstitution at sub-nanometer resolution. Lipid bilayer integrity and pro- tein activity were preserved upon the reconstitution process. Reversible struc- tural modifications of the membrane, induced by the bacteriorhodopsin func- tional activity triggered by visible light, were observed and characterized at the nanoscale.
LB  - PUB:(DE-HGF)16
C6  - pmid:33894545
UR  - <Go to ISI:>//WOS:000653055500004
DO  - DOI:10.1016/j.jcis.2021.03.155
UR  - https://juser.fz-juelich.de/record/903845
ER  -