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@ARTICLE{Mukhina:903845,
author = {Mukhina, Tetiana and Gerelli, Yuri and Hemmerle, Arnaud and
Koutsioumpas, Alexandros and Kovalev, Kirill and Teulon,
Jean-Marie and Pellequer, Jean-Luc and Daillant, Jean and
Charitat, Thierry and Fragneto, Giovanna},
title = {{I}nsertion and activation of functional
{B}acteriorhodopsin in a floating bilayer},
journal = {Journal of colloid and interface science},
volume = {597},
issn = {0021-9797},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2021-05478},
pages = {370 - 382},
year = {2021},
abstract = {The proton pump transmembrane protein bacteriorhodopsin was
successfully incorporated into planar floating lipid
bilayers in gel and fluid phases, by ap- plying a
detergent-mediated incorporation method. The method was
optimized on single supported bilayers by using quartz
crystal microbalance, atomic force and fluorescence
microscopy techniques. Neutron and X-ray reflectometry were
used on both single and floating bilayers with the aim of
determining the struc- ture and composition of this
membrane-protein system before and after protein
reconstitution at sub-nanometer resolution. Lipid bilayer
integrity and pro- tein activity were preserved upon the
reconstitution process. Reversible struc- tural
modifications of the membrane, induced by the
bacteriorhodopsin func- tional activity triggered by visible
light, were observed and characterized at the nanoscale.},
cin = {JCNS-FRM-II / JCNS-2 / JCNS-4 / MLZ},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-2-20110106 / I:(DE-Juel1)JCNS-4-20201012 /
I:(DE-588b)4597118-3},
pnm = {6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ)
(POF4-6G4) / 632 - Materials – Quantum, Complex and
Functional Materials (POF4-632)},
pid = {G:(DE-HGF)POF4-6G4 / G:(DE-HGF)POF4-632},
experiment = {EXP:(DE-MLZ)MARIA-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33894545},
UT = {WOS:000653055500004},
doi = {10.1016/j.jcis.2021.03.155},
url = {https://juser.fz-juelich.de/record/903845},
}