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@ARTICLE{Mukhina:903845,
      author       = {Mukhina, Tetiana and Gerelli, Yuri and Hemmerle, Arnaud and
                      Koutsioumpas, Alexandros and Kovalev, Kirill and Teulon,
                      Jean-Marie and Pellequer, Jean-Luc and Daillant, Jean and
                      Charitat, Thierry and Fragneto, Giovanna},
      title        = {{I}nsertion and activation of functional
                      {B}acteriorhodopsin in a floating bilayer},
      journal      = {Journal of colloid and interface science},
      volume       = {597},
      issn         = {0021-9797},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2021-05478},
      pages        = {370 - 382},
      year         = {2021},
      abstract     = {The proton pump transmembrane protein bacteriorhodopsin was
                      successfully incorporated into planar floating lipid
                      bilayers in gel and fluid phases, by ap- plying a
                      detergent-mediated incorporation method. The method was
                      optimized on single supported bilayers by using quartz
                      crystal microbalance, atomic force and fluorescence
                      microscopy techniques. Neutron and X-ray reflectometry were
                      used on both single and floating bilayers with the aim of
                      determining the struc- ture and composition of this
                      membrane-protein system before and after protein
                      reconstitution at sub-nanometer resolution. Lipid bilayer
                      integrity and pro- tein activity were preserved upon the
                      reconstitution process. Reversible struc- tural
                      modifications of the membrane, induced by the
                      bacteriorhodopsin func- tional activity triggered by visible
                      light, were observed and characterized at the nanoscale.},
      cin          = {JCNS-FRM-II / JCNS-2 / JCNS-4 / MLZ},
      ddc          = {540},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-2-20110106 / I:(DE-Juel1)JCNS-4-20201012 /
                      I:(DE-588b)4597118-3},
      pnm          = {6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ)
                      (POF4-6G4) / 632 - Materials – Quantum, Complex and
                      Functional Materials (POF4-632)},
      pid          = {G:(DE-HGF)POF4-6G4 / G:(DE-HGF)POF4-632},
      experiment   = {EXP:(DE-MLZ)MARIA-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33894545},
      UT           = {WOS:000653055500004},
      doi          = {10.1016/j.jcis.2021.03.155},
      url          = {https://juser.fz-juelich.de/record/903845},
}