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000904330 0247_ $$2ISSN$$a2041-6539
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000904330 1001_ $$00000-0002-2418-9421$$aMineev, Konstantin S.$$b0
000904330 245__ $$aNanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
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000904330 520__ $$aOne of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.
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000904330 7001_ $$0P:(DE-HGF)0$$aGoncharuk, Sergey A.$$b1
000904330 7001_ $$0P:(DE-HGF)0$$aGoncharuk, Marina V.$$b2
000904330 7001_ $$0P:(DE-HGF)0$$aPovarova, Natalia V.$$b3
000904330 7001_ $$0P:(DE-HGF)0$$aSokolov, Anatolii I.$$b4
000904330 7001_ $$0P:(DE-HGF)0$$aBaleeva, Nadezhda S.$$b5
000904330 7001_ $$00000-0002-1545-6604$$aSmirnov, Alexander Yu.$$b6
000904330 7001_ $$00000-0002-2168-3555$$aMyasnyanko, Ivan N.$$b7
000904330 7001_ $$0P:(DE-HGF)0$$aRuchkin, Dmitry A.$$b8
000904330 7001_ $$0P:(DE-HGF)0$$aBukhdruker, Sergey$$b9
000904330 7001_ $$0P:(DE-HGF)0$$aRemeeva, Alina$$b10
000904330 7001_ $$00000-0003-3759-380X$$aMishin, Alexey$$b11
000904330 7001_ $$0P:(DE-Juel1)191126$$aBorshchevskiy, Valentin$$b12$$ufzj
000904330 7001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b13$$ufzj
000904330 7001_ $$0P:(DE-HGF)0$$aArseniev, Alexander S.$$b14
000904330 7001_ $$0P:(DE-HGF)0$$aGorbachev, Dmitriy A.$$b15
000904330 7001_ $$0P:(DE-HGF)0$$aGavrikov, Alexey S.$$b16
000904330 7001_ $$00000-0002-4935-7030$$aMishin, Alexander S.$$b17
000904330 7001_ $$00000-0002-9339-7603$$aBaranov, Mikhail S.$$b18$$eCorresponding author
000904330 773__ $$0PERI:(DE-600)2559110-1$$a10.1039/D1SC01454D$$gVol. 12, no. 19, p. 6719 - 6725$$n19$$p6719 - 6725$$tChemical science$$v12$$x2041-6520$$y2021
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