Journal Article

FZJ-2021-05900

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids

Mineev, K. S. ; Goncharuk, S. A. ; Goncharuk, M. V. ; Povarova, N. V. ; Sokolov, A. I. ; Baleeva, N. S. ; Smirnov, A. Y. ; Myasnyanko, I. N. ; Ruchkin, D. A. ; Bukhdruker, S. ; Remeeva, A. ; Mishin, A. ; Borshchevskiy, V.FZJ* ; Gordeliy, V.FZJ* ; Arseniev, A. S. ; Gorbachev, D. A. ; Gavrikov, A. S. ; Mishin, A. S. ; Baranov, M. S. (Corresponding author)

2021
RSC Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/29695  doi:10.1039/D1SC01454D

Abstract: One of the essential characteristics of any tag used in bioscience and medical applications is its size. The larger the label, the more it may affect the studied object, and the more it may distort its behavior. In this paper, using NMR spectroscopy and X-ray crystallography, we have studied the structure of fluorogen-activating protein FAST both in the apo form and in complex with the fluorogen. We showed that significant change in the protein occurs upon interaction with the ligand. While the protein is completely ordered in the complex, its apo form is characterized by higher mobility and disordering of its N-terminus. We used structural information to design the shortened FAST (which we named nanoFAST) by truncating 26 N-terminal residues. Thus, we created the shortest genetically encoded tag among all known fluorescent and fluorogen-activating proteins, which is composed of only 98 amino acids.

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Contributing Institute(s):

1. Strukturbiochemie (IBI-7)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2021

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Database coverage:
; ; ; ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; IF >= 5 ; Index Chemicus ; JCR ; National-Konsortium ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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