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| Home > Publications database > Characterizing Root System Plasticity during Plant-Nematode Interaction Using Magnetic Resonance Imaging (MRI) |
| Home > Publications database > Characterizing Root System Plasticity during Plant-Nematode Interaction Using Magnetic Resonance Imaging (MRI) |
| Conference Presentation (After Call) | FZJ-2022-00407 |
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2021
Abstract: Damage caused by root-knot nematodes (RKNs) is highly remarkable for below-ground plant parts especially at the early stages of infection. Gall development at the roots is the primary symptom observed during RKN parasitism. The opaque nature of soils does not allow direct in-situ quantification of the dynamic response of roots to RKN infection. Accessing and quantifying such effects has been mostly based on destructive methods. In this current study, magnetic resonance imaging (MRI) was used to non-invasively observe the dynamic response of carrot roots infected with RKNs in soil-filled microcosms. We subjected RKN infected plants to MRI plant phenotyping platform at a 7-day interval until a stage of 49 days post inoculation (dpi). Acquired images were analyzed using the MeVisLab application software. Analysis of 3 dimensional images showed the initiation of gall development at 28 dpi and allowed the tempo-spatial analysis of gall development on roots until 49 dpi. Gall development was prominent on secondary roots and mostly within the uppermost 0.5 to 5 cm part of roots. Classification of gall sizes revealed that MRI allowed the detection of galls above 0.6 mm width. Further, the presence of RKN reduced taproot volume by 20%. These findings indicate the utility and capacity of MRI as a potent non-invasive approach for monitoring and quantifying early responses of below-ground plant parts to RKNs infection.
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