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@INPROCEEDINGS{Agyei:905115,
author = {Agyei, Kwabena M. and van Dusschoten, Dagmar and
Pflugfelder, Daniel and Schleker, A. Syliva and Koller,
Robert and Florian, M. W.},
title = {{C}haracterizing {R}oot {S}ystem {P}lasticity during
{P}lant-{N}ematode {I}nteraction {U}sing {M}agnetic
{R}esonance {I}maging ({MRI})},
reportid = {FZJ-2022-00407},
year = {2021},
abstract = {Damage caused by root-knot nematodes (RKNs) is highly
remarkable for below-ground plant parts especially at the
early stages of infection. Gall development at the roots is
the primary symptom observed during RKN parasitism. The
opaque nature of soils does not allow direct in-situ
quantification of the dynamic response of roots to RKN
infection. Accessing and quantifying such effects has been
mostly based on destructive methods. In this current study,
magnetic resonance imaging (MRI) was used to non-invasively
observe the dynamic response of carrot roots infected with
RKNs in soil-filled microcosms. We subjected RKN infected
plants to MRI plant phenotyping platform at a 7-day interval
until a stage of 49 days post inoculation (dpi). Acquired
images were analyzed using the MeVisLab application
software. Analysis of 3 dimensional images showed the
initiation of gall development at 28 dpi and allowed the
tempo-spatial analysis of gall development on roots until 49
dpi. Gall development was prominent on secondary roots and
mostly within the uppermost 0.5 to 5 cm part of roots.
Classification of gall sizes revealed that MRI allowed the
detection of galls above 0.6 mm width. Further, the presence
of RKN reduced taproot volume by $20\%.$ These findings
indicate the utility and capacity of MRI as a potent
non-invasive approach for monitoring and quantifying early
responses of below-ground plant parts to RKNs infection.},
month = {May},
date = {2021-05-24},
organization = {11th Symposium of the International
Society of Root Research, online (USA),
24 May 2021 - 28 May 2021},
subtyp = {After Call},
cin = {IBG-2},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {2171 - Biological and environmental resources for
sustainable use (POF4-217)},
pid = {G:(DE-HGF)POF4-2171},
typ = {PUB:(DE-HGF)6},
url = {https://juser.fz-juelich.de/record/905115},
}