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@INPROCEEDINGS{Macabuhay:908269,
      author       = {Macabuhay, Allene and Sanow, Stefan and Nagel, Kerstin and
                      Kuang, Weiqi and Lenz, Henning and Putz, Alexander and
                      Huesgen, Pitter and Roessner, Ute and Watt, Michelle and
                      Arsova, Borjana},
      title        = {{A}biotic condition plays an important role and shapes the
                      amplitude or the direction of the plant - microbe
                      interaction, on plant-phenotype and molecular level, in
                      studies of elevated temperature or nitrogen limitation},
      reportid     = {FZJ-2022-02503},
      year         = {2022},
      abstract     = {Plant roots grow in a changing environment in every stage
                      of soil exploration [1]. Studying the root in its native
                      conditions is challenging, but non-invasive phenotyping can
                      help to understand the space-time interaction between root
                      growth, soil and microbes [2].Elevated temperature is a
                      rising agricultural problem. We show that Paraburkholderia
                      phytofirmans (PsJN) improved plant performance in
                      Arabidopsis at 30°C, using an advanced version of
                      GrowScreen-Agar [3]. Root responses to PsJN were dynamic,
                      and included increases in lengths and growth rates of roots
                      and changes in root angles under both temperatures. The
                      onset of beneficial interaction was at day 12 post
                      inoculation and the growth promotion was greater in plants
                      with PsJN at ambient temperature vs. 30°C. Importantly, the
                      improvement at 30°C significantly reduced heat stress
                      responses. Analysis of shoot biomass correlated to the root
                      results. Lipidomic measurements will compare systemic
                      changes e.g. of membrane lipids.Overuse of fertilizers is a
                      serious environmental problem, and recent regulations limit
                      Nitrogen (N) fertilizers per unit arable land impacting
                      yield. Thus, we study the plant response to N-fixing
                      bacteria. In two projects, the cereal model Brachypodium
                      dystachion (Bd) shows different response to N-fixing
                      bacteria based on N availability. Inoculation with
                      Herbaspirillum seropedicae (Hs) in EcoFABs [4] resulted in
                      longer primary roots and shorter root hairs regardless of N,
                      with stronger changes at low N. A mass-balance calculation
                      showed that at high N, Hs provided $11\%$ total plant N from
                      sources other than seed or nutrient solution, correlated to
                      N-metabolism transcript expression. Time-resolved phenotypic
                      and molecular data indicates two modes of action: At 5 mM Bd
                      benefits through Hs N-fixation; at 0.5 mM Hs promotes
                      N-uptake from the root medium.},
      month         = {Jul},
      date          = {2022-07-11},
      organization  = {MICROBE-ASSISTED CROP PRODUCTION
                       OPPORTUNITIES, CHALLENGES $\&$ NEEDS,
                       Vienna (Austria), 11 Jul 2022 - 14 Jul
                       2022},
      subtyp        = {Invited},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {2171 - Biological and environmental resources for
                      sustainable use (POF4-217)},
      pid          = {G:(DE-HGF)POF4-2171},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/908269},
}