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000916056 0247_ $$2doi$$a10.1021/acschemneuro.2c00465
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000916056 1001_ $$0P:(DE-HGF)0$$aRafiei, Yasin$$b0
000916056 245__ $$aPolyphenols-Based Nanosheets of Propolis Modulate Cytotoxic Amyloid Fibril Assembly of α-Synuclein
000916056 260__ $$aWashington, DC$$bACS Publ.$$c2022
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000916056 520__ $$aNatural compounds with anti-aggregation capacity are increasingly recognized as viable candidates against neurodegenerative diseases. Recently, the polyphenolic fraction of propolis (PFP), a complex bee product, has been shown to inhibit amyloid aggregation of a model protein especially in the nanosheet form. Here, we examine the aggregation-modulating effects of the PFP nanosheets on α-synuclein (α-syn), an intrinsically disordered protein involved in the pathogenesis of Parkinson's disease. Based on a range of biophysical data including intrinsic and extrinsic fluorescence, circular dichroism (CD) data, and nuclear magnetic resonance spectroscopy, we propose a model for the interaction of α-syn with PFP nanosheets, where the positively charged N-terminal and the middle non-amyloid component regions of α-syn act as the main binding sites with the negatively charged PFP nanosheets. The Thioflavin T (ThT) fluorescence, Congo red absorbance, and CD data reveal a prominent dose-dependent inhibitory effect of PFP nanosheets on α-syn amyloid aggregation, and the microscopy images and MTT assay data suggest that the PFP nanosheets redirect α-syn aggregation toward nontoxic off-pathway oligomers. When preformed α-syn amyloid fibrils are present, fluorescence images show co-localization of PFP nanosheets and ThT, further confirming the binding of PFP nanosheets with α-syn amyloid fibrils. Taken together, our results demonstrate the binding and anti-aggregation activity of PFP nanosheets in a disease-related protein system and propose them as potential nature-based tools for probing and targeting pathological protein aggregates in neurodegenerative diseases.
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000916056 7001_ $$0P:(DE-HGF)0$$aSalmani, Bahram$$b1
000916056 7001_ $$0P:(DE-HGF)0$$aMirzaei-Behbahani, Behnaz$$b2
000916056 7001_ $$0P:(DE-HGF)0$$aTaleb, Mahshid$$b3
000916056 7001_ $$00000-0002-1428-3254$$aMeratan, Ali Akbar$$b4$$eCorresponding author
000916056 7001_ $$0P:(DE-HGF)0$$aRamezani, Mohammad$$b5
000916056 7001_ $$00000-0002-5995-7734$$aNikfarjam, Nasser$$b6
000916056 7001_ $$0P:(DE-HGF)0$$aBecker, Stefan$$b7
000916056 7001_ $$0P:(DE-Juel1)194492$$aRezaie Ghaleh, Nasrollah$$b8$$eCorresponding author
000916056 773__ $$0PERI:(DE-600)2528493-9$$a10.1021/acschemneuro.2c00465$$gVol. 13, no. 22, p. 3168 - 3179$$n22$$p3168 - 3179$$tACS chemical neuroscience$$v13$$x1948-7193$$y2022
000916056 8564_ $$uhttps://juser.fz-juelich.de/record/916056/files/Polyphenols-Based%20Nanosheets%20of%20Propolis%20Modulate%20Cytotoxic%20Amyloid%20Fibril%20Assembly%20of%20%CE%B1-Synuclein-1.pdf
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