TY  - JOUR
AU  - Sehl, Torsten
AU  - Seibt, Lisa
AU  - Kappauf, Katrin
AU  - Ergezinger, Pia
AU  - Spöring, Jan-Dirk
AU  - Mielke, Kristina
AU  - Doeker, Moritz
AU  - Verma, Neha
AU  - Bocola, Marco
AU  - Daußmann, Thomas
AU  - Chen, Haibin
AU  - Shi, Shumin
AU  - Jupke, Andreas
AU  - Rother, Dörte
TI  - Enzymatic (2 R ,4 R )‐Pentanediol Synthesis – “Putting a Bottle on the Table”
JO  - Chemie - Ingenieur - Technik
VL  - 95
IS  - 4
SN  - 0009-286X
CY  - Weinheim
PB  - Wiley-VCH Verl.
M1  - FZJ-2023-00721
SP  - 557-564
PY  - 2023
AB  - (2R,4R)-Pentanediol is an interesting precursor for the synthesis of chiral ligands. A ketoreductase (KRED) was employed for the asymmetric reduction of acetylacetone to this diol. Biocatalysis often suffers from low concentrations of hydrophobic substrates and low stability of the enzyme in unconventional media. Here, we present an engineered KRED variant applicable in a neat substrate system, including upscaling to the multi-liter scale and downstream processing (DSP). Our engineered KRED applied in a neat substrate system is a powerful technique for the synthesis of chiral diols yielding product concentrations of 208 g L−1.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000914001400001
DO  - DOI:10.1002/cite.202200178
UR  - https://juser.fz-juelich.de/record/917508
ER  -