Journal Article

FZJ-2023-00721

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Enzymatic (2 R ,4 R )‐Pentanediol Synthesis – “Putting a Bottle on the Table”

Sehl, T. (Corresponding author)FZJ* ; Seibt, L.FZJ* ; Kappauf, K. ; Ergezinger, P. ; Spöring, J.-D.FZJ* ; Mielke, K. ; Doeker, M. ; Verma, N. ; Bocola, M. ; Daußmann, T. ; Chen, H. ; Shi, S. ; Jupke, A.FZJ* ; Rother, D. (Corresponding author)FZJ*

2023
Wiley-VCH Verl. Weinheim

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Please use a persistent id in citations: http://hdl.handle.net/2128/34204  doi:10.1002/cite.202200178

Abstract: (2R,4R)-Pentanediol is an interesting precursor for the synthesis of chiral ligands. A ketoreductase (KRED) was employed for the asymmetric reduction of acetylacetone to this diol. Biocatalysis often suffers from low concentrations of hydrophobic substrates and low stability of the enzyme in unconventional media. Here, we present an engineered KRED variant applicable in a neat substrate system, including upscaling to the multi-liter scale and downstream processing (DSP). Our engineered KRED applied in a neat substrate system is a powerful technique for the synthesis of chiral diols yielding product concentrations of 208 g L−1.

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2023

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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