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000943370 0247_ $$2doi$$a10.1007/978-1-0716-2329-9_6
000943370 0247_ $$2ISSN$$a1064-3745
000943370 0247_ $$2ISSN$$a1940-6029
000943370 037__ $$aFZJ-2023-00969
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000943370 1001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b0$$eEditor$$ufzj
000943370 245__ $$aCrystallization of Microbial Rhodopsins
000943370 260__ $$aNew York, NY$$bSpringer US$$c2022
000943370 29510 $$aRhodopsin / Gordeliy, Valentin (Editor) ; New York, NY : Springer US, 2022, Chapter 6 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2328-2=978-1-0716-2329-9 ; doi:10.1007/978-1-0716-2329-9
000943370 300__ $$a125 - 146
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000943370 4900_ $$aMethods in Molecular Biology$$v2501
000943370 520__ $$aMicrobial rhodopsins are light-sensitive transmembrane proteins, evolutionary adapted by various organisms like archaea, bacteria, simple eukaryote, and viruses to utilize solar energy for their survival. A complete understanding of functional mechanisms of these proteins is not possible without the knowledge of their high-resolution structures, which can be primarily obtained by X-ray crystallography. This technique, however, requires high-quality crystals, growing of which is a great challenge especially in case of membrane proteins. In this chapter, we summarize methods applied for crystallization of microbial rhodopsins with the emphasis on crystallization in lipidic mesophases, also known as in meso approach. In particular, we describe in detail the methods of crystallization using lipidic cubic phase to grow both large crystals optimized for traditional crystallographic data collection and microcrystals for serial crystallography.
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000943370 650_0 $$aRhodopsin
000943370 7001_ $$aKovalev, Kirill$$b1
000943370 7001_ $$0P:(DE-HGF)0$$aAstashkin, Roman$$b2
000943370 7001_ $$0P:(DE-Juel1)131964$$aGordeliy, Valentin$$b3$$eCorresponding author$$ufzj
000943370 7001_ $$0P:(DE-HGF)0$$aCherezov, Vadim$$b4
000943370 773__ $$a10.1007/978-1-0716-2329-9_6
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000943370 9141_ $$y2022
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