Contribution to a book

FZJ-2023-00969

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Crystallization of Microbial Rhodopsins

Gordeliy, V. (Editor)FZJ* ; Kovalev, K. ; Astashkin, R. ; Gordeliy, V. (Corresponding author)FZJ* ; Cherezov, V.

2022
Springer US New York, NY
ISBN: 978-1-0716-2328-2, 978-1-0716-2329-9 (electronic)

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Please use a persistent id in citations: doi:10.1007/978-1-0716-2329-9_6

Abstract: Microbial rhodopsins are light-sensitive transmembrane proteins, evolutionary adapted by various organisms like archaea, bacteria, simple eukaryote, and viruses to utilize solar energy for their survival. A complete understanding of functional mechanisms of these proteins is not possible without the knowledge of their high-resolution structures, which can be primarily obtained by X-ray crystallography. This technique, however, requires high-quality crystals, growing of which is a great challenge especially in case of membrane proteins. In this chapter, we summarize methods applied for crystallization of microbial rhodopsins with the emphasis on crystallization in lipidic mesophases, also known as in meso approach. In particular, we describe in detail the methods of crystallization using lipidic cubic phase to grow both large crystals optimized for traditional crystallographic data collection and microcrystals for serial crystallography.

Keyword(s): Rhodopsin (LCSH)

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Contributing Institute(s):

1. Strukturbiochemie (IBI-7)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2022

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Database coverage:
; SCOPUS

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