TY  - CHAP
AU  - Kovalev, Kirill
AU  - Astashkin, Roman
AU  - Gordeliy, Valentin
AU  - Cherezov, Vadim
A3  - Gordeliy, Valentin
TI  - Crystallization of Microbial Rhodopsins
VL  - 2501
CY  - New York, NY
PB  - Springer US
M1  - FZJ-2023-00969
SN  - 978-1-0716-2328-2
T2  - Methods in Molecular Biology
SP  - 125 - 146
PY  - 2022
AB  - Microbial rhodopsins are light-sensitive transmembrane proteins, evolutionary adapted by various organisms like archaea, bacteria, simple eukaryote, and viruses to utilize solar energy for their survival. A complete understanding of functional mechanisms of these proteins is not possible without the knowledge of their high-resolution structures, which can be primarily obtained by X-ray crystallography. This technique, however, requires high-quality crystals, growing of which is a great challenge especially in case of membrane proteins. In this chapter, we summarize methods applied for crystallization of microbial rhodopsins with the emphasis on crystallization in lipidic mesophases, also known as in meso approach. In particular, we describe in detail the methods of crystallization using lipidic cubic phase to grow both large crystals optimized for traditional crystallographic data collection and microcrystals for serial crystallography.
LB  - PUB:(DE-HGF)7
DO  - DOI:10.1007/978-1-0716-2329-9_6
UR  - https://juser.fz-juelich.de/record/943370
ER  -