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@INBOOK{Gordeliy:943370,
author = {Kovalev, Kirill and Astashkin, Roman and Gordeliy, Valentin
and Cherezov, Vadim},
editor = {Gordeliy, Valentin},
title = {{C}rystallization of {M}icrobial {R}hodopsins},
volume = {2501},
address = {New York, NY},
publisher = {Springer US},
reportid = {FZJ-2023-00969},
isbn = {978-1-0716-2328-2},
series = {Methods in Molecular Biology},
pages = {125 - 146},
year = {2022},
comment = {Rhodopsin / Gordeliy, Valentin (Editor) ; New York, NY :
Springer US, 2022, Chapter 6 ; ISSN: 1064-3745=1940-6029 ;
ISBN: 978-1-0716-2328-2=978-1-0716-2329-9 ;
doi:10.1007/978-1-0716-2329-9},
booktitle = {Rhodopsin / Gordeliy, Valentin
(Editor) ; New York, NY : Springer US,
2022, Chapter 6 ; ISSN:
1064-3745=1940-6029 ; ISBN:
978-1-0716-2328-2=978-1-0716-2329-9 ;
doi:10.1007/978-1-0716-2329-9},
abstract = {Microbial rhodopsins are light-sensitive transmembrane
proteins, evolutionary adapted by various organisms like
archaea, bacteria, simple eukaryote, and viruses to utilize
solar energy for their survival. A complete understanding of
functional mechanisms of these proteins is not possible
without the knowledge of their high-resolution structures,
which can be primarily obtained by X-ray crystallography.
This technique, however, requires high-quality crystals,
growing of which is a great challenge especially in case of
membrane proteins. In this chapter, we summarize methods
applied for crystallization of microbial rhodopsins with the
emphasis on crystallization in lipidic mesophases, also
known as in meso approach. In particular, we describe in
detail the methods of crystallization using lipidic cubic
phase to grow both large crystals optimized for traditional
crystallographic data collection and microcrystals for
serial crystallography.},
cin = {IBI-7},
ddc = {570},
cid = {I:(DE-Juel1)IBI-7-20200312},
pnm = {5241 - Molecular Information Processing in Cellular Systems
(POF4-524)},
pid = {G:(DE-HGF)POF4-5241},
typ = {PUB:(DE-HGF)7},
doi = {10.1007/978-1-0716-2329-9_6},
url = {https://juser.fz-juelich.de/record/943370},
}