Contribution to a book FZJ-2023-00970

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E. coli Expression and Purification of Microbial and Viral Rhodopsins

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2022
Springer US New York, NY
ISBN: 978-1-0716-2328-2, 978-1-0716-2329-9 (electronic)

Rhodopsin / Gordeliy, Valentin (Editor) ; New York, NY : Springer US, 2022, Chapter 5 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2328-2=978-1-0716-2329-9 ; doi:10.1007/978-1-0716-2329-9 New York, NY : Springer US, Methods in Molecular Biology 2501, 109 - 124 () [10.1007/978-1-0716-2329-9_5]

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Abstract: Microbial rhodopsins have become an indispensable tool for neurobiology. Of thousands of identified microbial rhodopsins, a minute fraction has been studied so far and they have shown remarkable functional diversity suggesting more great promises that this large family holds. Effective production of recombinant microbial and viral rhodopsins is a prerequisite for the success of functional and structural studies of these proteins. Escherichia coli (E. coli) are suitable for high yield expression of many of microbial and viral rhodopsins and they facilitate rapid exploration of this large protein family.

Classification:

Contributing Institute(s):
  1. Strukturbiochemie (IBI-7)
Research Program(s):
  1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)
  2. DFG project 430170559 - Strukturelle Grundlagen der Signalweiterleitung in der Phototaxis von Archaeen (430170559) (430170559)

Appears in the scientific report 2022
Database coverage:
Medline ; SCOPUS
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 Datensatz erzeugt am 2023-01-24, letzte Änderung am 2023-02-28



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