Poster (Other)

FZJ-2023-01041

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Real-time investigation of Lysozyme crystallization kinetics: a neutron diffractionstudy

Longo, M. ; Heigl, R. ; Schrader, T. E. (Corresponding author)FZJ*

2022

Please use a persistent id in citations: http://hdl.handle.net/2128/33889

Abstract: The isothermal crystallization kinetics of hen-egg-white Lysozyme has been investigated by means of a time-resolved neutron diffraction experiment for almost 3 days, starting from a supersaturated solution of Lysozyme (30 mg/ml, 3 wt % NaCl, pD 4.75 at 298 K) until the growth of crystals, in order to have complementary information about the crystallization kinetics1. The temporal evolution of the intensity of the Bragg peaks, observed in the neutron diffraction images when a single crystal appears, has been studied. Simultaneously, the analysis of the small angle neutron scattering curves from the Lysozyme solution, during the crystallization process, has been performed. A correlated behaviour between the decrease of the Lysozyme concentration and the increase of the crystallization fraction was observed. The crystallization kinetics was described by means of the Johnson-Mehl-Avrami-Kolmogorov model and parameters compatible with our previous Lysozyme crystallization study have been found2. The Lysozyme crystallization under constant neutron flux underlines the known strength of neutrons in studying biological samples without causing radiation damage.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. JCNS-4 (JCNS-4)
3. Heinz Maier-Leibnitz Zentrum (MLZ)
4. Neutronenstreuung (JCNS-1)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. BIODIFF: Diffractometer for large unit cells (NL1)

Appears in the scientific report 2022

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Database coverage:

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