Journal Article

FZJ-2023-01594

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase

Muñoz, P. ; Castillejo, C. ; Gómez, J. A. ; Miranda, L. ; Lesemann, S. ; Olbricht, K. ; Petit, A. ; Chartier, P. ; Haugeneder, A. ; Trinkl, J. ; Mazzoni, L. ; Masny, A. ; Zurawicz, E. ; Ziegler, F. M. R.FZJ* ; Usadel, B.FZJ* ; Schwab, W. ; Denoyes, B. ; Mezzetti, B. ; Osorio, S. ; Sánchez-Sevilla, J. F. ; Amaya, I. (Corresponding author)

2023
Nature Publ. Group London

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Please use a persistent id in citations: http://hdl.handle.net/2128/34208  doi:10.1093/hr/uhad006

Abstract: Strawberry (Fragaria × ananassa) fruits are an excellent source of L-ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines ‘Candonga’ and ‘Senga Sengana’, adapted to distinct Southern and Northern European areas. To account for environmental effects, the F1 and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A), and the chloroplast-located AsA transporter gene FaPHT4;4(7C) might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of FaGGP(3A) inherited from both parental lines increase fruit AsA content. Furthermore, expression of FaGGP(3A) was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.

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Contributing Institute(s):

1. Bioinformatik (IBG-4)

Research Program(s):

1. 2171 - Biological and environmental resources for sustainable use (POF4-217) (POF4-217)
2. GoodBerry - Improving the stability of high-quality traits of berry in different environments and cultivation systems for the benefit of European farmers and consumers (679303) (679303)

Appears in the scientific report 2023

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