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| Home > Publications database > Analysing the Effect of Noise on Static and Dynamic Results from Total Internal Reflection Microscopy (TIRM) |
| Conference Presentation (After Call) | FZJ-2025-04319 |
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2025
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Please use a persistent id in citations: doi:10.34734/FZJ-2025-04319
Abstract: Total internal reflection microscopy (TIRM) has been used for about 30 years to measure interaction potentials between suspended spherical colloids and flat surfaces. The method also allows measuring position resolved dynamic properties, specifically the diffusion coefficient and the drift velocity normal to the surface. However, in literature there are reports on dynamic results which are at conflict with expectations from standard hydrodynamics. In this work, we have used experimental TIRM in conjunction with molecular dynamics simulations to investigate the effects of inherent error sources due to noise artifacts on TIRM results. We find that detector shot noise and too long sampling times may cause erroneous results in the steep parts of the interaction potential. Further, the noise does not significantly affect dynamic data, but we find that too long sampling times and/or too small probe particles will cause issues. Additionally, we observe that dynamic results are very likely to differ from the standard hydrodynamic predictions for stick boundary conditions. Thus, to minimize experimental errors, sampling times and particle sizes have to be properly chosen when designing TIRM experiments and for the analysis of dynamic information, partial slip conditions have to be considered.
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