Journal Article

FZJ-2013-04404

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium

Unthan, S. (Corresponding author)FZJ* ; Grünberger, A.FZJ* ; van Ooyen, J.FZJ* ; Gätgens, J.FZJ* ; Heinrich, J.FZJ* ; Paczia, N.FZJ* ; Wiechert, W.FZJ* ; Kohlheyer, D.FZJ* ; Noack, S. (Corresponding Author)FZJ*

2014
Wiley New York, NY [u.a.]

This record in other databases:    

Please use a persistent id in citations: doi:10.1002/bit.25103

Abstract: In a former study we showed that Corynebacterium glutamicum grows much faster in defined CGXII glucose medium when growth was initiated in highly diluted environments [Grünberger et al. (2013b) Biotechnol Bioeng]. Here we studied the batch growth of C. glutamicum in CGXII at a comparable low starting biomass concentration of OD ≈ 0.005 in more detail. During bioreactor cultivations a bi-phasic growth behavior with changing growth rates was observed. Initially the culture grew with inline image before the growth rate dropped to inline image. We were able to confirm the elevated growth rate for C. glutamicum in CGXII and showed for the first time a growth rate beyond 0.6 in lab-scale bioreactor cultivations on defined medium. Advanced growth studies combining well-designed bioreactor and microfluidic single-cell cultivations (MSCC) with quantitative transcriptomics, metabolomics and integrative in silico analysis revealed protocatechuic acid as a hidden co-substrate for accelerated growth within CGXII. The presented approach proves the general applicability of MSCC to investigate and validate the effect of single medium components on microorganism growth during cultivation in liquid media, and therefore might be of interest for any kind of basic growth study

---

Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 899 - ohne Topic (POF2-899) (POF2-899)

Appears in the scientific report 2014

---

Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---