Journal Article

FZJ-2014-01177

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Femtosecond X-ray protein nanocrystallography

Chapman, H. N. (Corresponding author) ; Fromme, P. ; Barty, A. ; White, T. A. ; Kirian, R. A. ; Aquila, A. ; Hunter, M. S. ; Schulz, J. ; DePonte, D. P. ; Weierstall, U. ; Doak, R. B. ; Maia, F. R. N. C. ; Martin, A. V. ; Schlichting, I. ; Lomb, L. ; Coppola, N. ; Shoeman, R. L. ; Epp, S. W. ; Hartmann, R. ; Rolles, D. ; Rudenko, A. ; Foucar, L. ; Kimmel, N. ; Weidenspointner, G. ; Holl, P. ; Liang, M. ; Barthelmess, M. ; Caleman, C. ; Boutet, S. ; Bogan, M. J. ; Krzywinski, J. ; Bostedt, C. ; Bajt, S. ; Gumprecht, L. ; Rudek, B. ; Erk, B. ; Schmidt, C. ; Hömke, A. ; Reich, C. ; Pietschner, D. ; Strüder, L. ; Hauser, G. ; Gorke, H.ZEA-2* ; Ullrich, J. ; Herrmann, S. ; Schaller, G. ; Schopper, F. ; Soltau, H. ; Kühnel, K.-U. ; Messerschmidt, M. ; Bozek, J. D. ; Hau-Riege, S. P. ; Frank, M. ; Hampton, C. Y. ; Sierra, R. G. ; Starodub, D. ; Williams, G. J. ; Hajdu, J. ; Timneanu, N. ; Seibert, M. M. ; Andreasson, J. ; Rocker, A. ; Jönsson, O. ; Svenda, M. ; Stern, S. ; Nass, K. ; Andritschke, R. ; Schröter, C.-D. ; Krasniqi, F. ; Bott, M. ; Schmidt, K. E. ; Wang, X. ; Grotjohann, I. ; Holton, J. M. ; Barends, T. R. M. ; Neutze, R. ; Marchesini, S. ; Fromme, R. ; Schorb, S. ; Rupp, D. ; Adolph, M. ; Gorkhover, T. ; Andersson, I. ; Hirsemann, H. ; Potdevin, G. ; Graafsma, H. ; Nilsson, B. ; Spence, J. C. H.

2011
Nature Publising Group London [u.a.]

This record in other databases:      

Please use a persistent id in citations: doi:10.1038/nature09750

Abstract: X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded1, 2, 3. It is particularly challenging to obtain large, well-diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single-crystal X-ray diffraction ‘snapshots’ are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source4. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes5. More than 3,000,000 diffraction patterns were collected in this study, and a three-dimensional data set was assembled from individual photosystem I nanocrystals (~200 nm to 2 μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes6. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage.

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Contributing Institute(s):

1. Zentralinstitut für Elektronik (ZEA-2)

Research Program(s):

1. 531 - Hadron Structure and Dynamics (HSD) (POF2-531) (POF2-531)

Appears in the scientific report 2014

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